Available from Reaxense
This protein is integrated into the Receptor.AI ecosystem as a prospective target with high therapeutic potential. We performed a comprehensive characterization of Netrin receptor UNC5C including:
1. LLM-powered literature research
Our custom-tailored LLM extracted and formalized all relevant information about the protein from a large set of structured and unstructured data sources and stored it in the form of a Knowledge Graph. This comprehensive analysis allowed us to gain insight into Netrin receptor UNC5C therapeutic significance, existing small molecule ligands, relevant off-targets, and protein-protein interactions.
Fig. 1. Preliminary target research workflow
2. AI-Driven Conformational Ensemble Generation
Starting from the initial protein structure, we employed advanced AI algorithms to predict alternative functional states of Netrin receptor UNC5C, including large-scale conformational changes along "soft" collective coordinates. Through molecular simulations with AI-enhanced sampling and trajectory clustering, we explored the broad conformational space of the protein and identified its representative structures. Utilizing diffusion-based AI models and active learning AutoML, we generated a statistically robust ensemble of equilibrium protein conformations that capture the receptor's full dynamic behavior, providing a robust foundation for accurate structure-based drug design.
Fig. 2. AI-powered molecular dynamics simulations workflow
3. Binding pockets identification and characterization
We employed the AI-based pocket prediction module to discover orthosteric, allosteric, hidden, and cryptic binding pockets on the protein’s surface. Our technique integrates the LLM-driven literature search and structure-aware ensemble-based pocket detection algorithm that utilizes previously established protein dynamics. Tentative pockets are then subject to AI scoring and ranking with simultaneous detection of false positives. In the final step, the AI model assesses the druggability of each pocket enabling a comprehensive selection of the most promising pockets for further targeting.
Fig. 3. AI-based binding pocket detection workflow
4. AI-Powered Virtual Screening
Our ecosystem is equipped to perform AI-driven virtual screening on Netrin receptor UNC5C. With access to a vast chemical space and cutting-edge AI docking algorithms, we can rapidly and reliably predict the most promising, novel, diverse, potent, and safe small molecule ligands of Netrin receptor UNC5C. This approach allows us to achieve an excellent hit rate and to identify compounds ready for advanced lead discovery and optimization.
Fig. 4. The screening workflow of Receptor.AI
Receptor.AI, in partnership with Reaxense, developed a next-generation technology for on-demand focused library design to enable extensive target exploration.
The focused library for Netrin receptor UNC5C includes a list of the most effective modulators, each annotated with 38 ADME-Tox and 32 physicochemical and drug-likeness parameters. Furthermore, each compound is shown with its optimal docking poses, affinity scores, and activity scores, offering a detailed summary.
Netrin receptor UNC5C
partner:
Reaxense
upacc:
O95185
UPID:
UNC5C_HUMAN
Alternative names:
Protein unc-5 homolog 3; Protein unc-5 homolog C
Alternative UPACC:
O95185; Q8IUT0
Background:
Netrin receptor UNC5C, also known as Protein unc-5 homolog 3 or C, plays a pivotal role in axon guidance by mediating axon repulsion in the developing nervous system. It interacts with NTN1/Netrin-1, influencing microtubule dynamics and axon repulsion. Additionally, UNC5C is involved in corticospinal tract axon guidance and dorsal root ganglion axon projection, acting as a dependence receptor for apoptosis induction in the absence of netrin ligand.
Therapeutic significance:
Given its involvement in Alzheimer's disease, characterized by neurodegeneration and amyloid-beta protein deposition, UNC5C's understanding could pave the way for innovative therapeutic strategies targeting neurodegenerative disorders.