AI-ACCELERATED DRUG DISCOVERY
Available from Reaxense
This protein is integrated into the Receptor.AI ecosystem as a prospective target with high therapeutic potential. We performed a comprehensive characterization of Tubulin polyglutamylase TTLL5 including:
1. LLM-powered literature research
Our custom-tailored LLM extracted and formalized all relevant information about the protein from a large set of structured and unstructured data sources and stored it in the form of a Knowledge Graph. This comprehensive analysis allowed us to gain insight into Tubulin polyglutamylase TTLL5 therapeutic significance, existing small molecule ligands, relevant off-targets, and protein-protein interactions.
Fig. 1. Preliminary target research workflow
2. AI-Driven Conformational Ensemble Generation
Starting from the initial protein structure, we employed advanced AI algorithms to predict alternative functional states of Tubulin polyglutamylase TTLL5, including large-scale conformational changes along "soft" collective coordinates. Through molecular simulations with AI-enhanced sampling and trajectory clustering, we explored the broad conformational space of the protein and identified its representative structures. Utilizing diffusion-based AI models and active learning AutoML, we generated a statistically robust ensemble of equilibrium protein conformations that capture the receptor's full dynamic behavior, providing a robust foundation for accurate structure-based drug design.
Fig. 2. AI-powered molecular dynamics simulations workflow
3. Binding pockets identification and characterization
We employed the AI-based pocket prediction module to discover orthosteric, allosteric, hidden, and cryptic binding pockets on the protein’s surface. Our technique integrates the LLM-driven literature search and structure-aware ensemble-based pocket detection algorithm that utilizes previously established protein dynamics. Tentative pockets are then subject to AI scoring and ranking with simultaneous detection of false positives. In the final step, the AI model assesses the druggability of each pocket enabling a comprehensive selection of the most promising pockets for further targeting.
Fig. 3. AI-based binding pocket detection workflow
4. AI-Powered Virtual Screening
Our ecosystem is equipped to perform AI-driven virtual screening on Tubulin polyglutamylase TTLL5. With access to a vast chemical space and cutting-edge AI docking algorithms, we can rapidly and reliably predict the most promising, novel, diverse, potent, and safe small molecule ligands of Tubulin polyglutamylase TTLL5. This approach allows us to achieve an excellent hit rate and to identify compounds ready for advanced lead discovery and optimization.
Fig. 4. The screening workflow of Receptor.AI
Receptor.AI, in partnership with Reaxense, developed a next-generation technology for on-demand focused library design to enable extensive target exploration.
The focused library for Tubulin polyglutamylase TTLL5 includes a list of the most effective modulators, each annotated with 38 ADME-Tox and 32 physicochemical and drug-likeness parameters. Furthermore, each compound is shown with its optimal docking poses, affinity scores, and activity scores, offering a detailed summary.
Tubulin polyglutamylase TTLL5
partner:
Reaxense
upacc:
Q6EMB2
UPID:
TTLL5_HUMAN
Alternative names:
SRC1 and TIF2-associated modulatory protein; Tubulin--tyrosine ligase-like protein 5
Alternative UPACC:
Q6EMB2; B9EGH8; B9EGH9; Q9BUB0; Q9H0G4; Q9H7W2; Q9P1V5; Q9UPZ4
Background:
Tubulin polyglutamylase TTLL5, also known as SRC1 and TIF2-associated modulatory protein, plays a crucial role in modifying tubulin. This enzyme generates polyglutamate side chains on tubulin, essential for proper cellular function. It shows a preference for initiating over elongating the polyglutamylation reaction and modifies alpha-tubulin more than beta-tubulin. TTLL5 is vital for CCSAP localization to microtubules and enhances NCOA2/TIF2's effects in hormone receptor signaling.
Therapeutic significance:
TTLL5's mutation is linked to Cone-rod dystrophy 19, a retinal dystrophy leading to severe vision loss. Understanding TTLL5's role could unveil new therapeutic strategies for this and potentially other related diseases.
