Available from Reaxense
This protein is integrated into the Receptor.AI ecosystem as a prospective target with high therapeutic potential. We performed a comprehensive characterization of SMC5-SMC6 complex localization factor protein 2 including:
1. LLM-powered literature research
Our custom-tailored LLM extracted and formalized all relevant information about the protein from a large set of structured and unstructured data sources and stored it in the form of a Knowledge Graph. This comprehensive analysis allowed us to gain insight into SMC5-SMC6 complex localization factor protein 2 therapeutic significance, existing small molecule ligands, relevant off-targets, and protein-protein interactions.
Fig. 1. Preliminary target research workflow
2. AI-Driven Conformational Ensemble Generation
Starting from the initial protein structure, we employed advanced AI algorithms to predict alternative functional states of SMC5-SMC6 complex localization factor protein 2, including large-scale conformational changes along "soft" collective coordinates. Through molecular simulations with AI-enhanced sampling and trajectory clustering, we explored the broad conformational space of the protein and identified its representative structures. Utilizing diffusion-based AI models and active learning AutoML, we generated a statistically robust ensemble of equilibrium protein conformations that capture the receptor's full dynamic behavior, providing a robust foundation for accurate structure-based drug design.
Fig. 2. AI-powered molecular dynamics simulations workflow
3. Binding pockets identification and characterization
We employed the AI-based pocket prediction module to discover orthosteric, allosteric, hidden, and cryptic binding pockets on the protein’s surface. Our technique integrates the LLM-driven literature search and structure-aware ensemble-based pocket detection algorithm that utilizes previously established protein dynamics. Tentative pockets are then subject to AI scoring and ranking with simultaneous detection of false positives. In the final step, the AI model assesses the druggability of each pocket enabling a comprehensive selection of the most promising pockets for further targeting.
Fig. 3. AI-based binding pocket detection workflow
4. AI-Powered Virtual Screening
Our ecosystem is equipped to perform AI-driven virtual screening on SMC5-SMC6 complex localization factor protein 2. With access to a vast chemical space and cutting-edge AI docking algorithms, we can rapidly and reliably predict the most promising, novel, diverse, potent, and safe small molecule ligands of SMC5-SMC6 complex localization factor protein 2. This approach allows us to achieve an excellent hit rate and to identify compounds ready for advanced lead discovery and optimization.
Fig. 4. The screening workflow of Receptor.AI
Receptor.AI, in partnership with Reaxense, developed a next-generation technology for on-demand focused library design to enable extensive target exploration.
The focused library for SMC5-SMC6 complex localization factor protein 2 includes a list of the most effective modulators, each annotated with 38 ADME-Tox and 32 physicochemical and drug-likeness parameters. Furthermore, each compound is shown with its optimal docking poses, affinity scores, and activity scores, offering a detailed summary.
SMC5-SMC6 complex localization factor protein 2
partner:
Reaxense
upacc:
Q8IX21
UPID:
SLF2_HUMAN
Alternative names:
Smc5/6 localization factor 1
Alternative UPACC:
Q8IX21; A8K950; B1AL17; Q5W0L8; Q6GMU6; Q9NPE8
Background:
The SMC5-SMC6 complex localization factor protein 2, also known as Smc5/6 localization factor 1, is pivotal in the DNA damage response (DDR) pathway. It regulates postreplication repair of UV-damaged DNA and maintains genomic stability. This protein, through the SLF1-SLF2 complex, links RAD18 with the SMC5-SMC6 complex at replication-coupled interstrand cross-links and DNA double-strand breaks sites during DNA repair.
Therapeutic significance:
Given its crucial role in DNA repair and genomic stability, understanding the role of SMC5-SMC6 complex localization factor protein 2 could open doors to potential therapeutic strategies for Atelis syndrome 1, a disorder marked by developmental delay, microcephaly, and various physical anomalies, where patient cells exhibit spontaneous chromosome breakage.