AI-ACCELERATED DRUG DISCOVERY
Available from Reaxense
This protein is integrated into the Receptor.AI ecosystem as a prospective target with high therapeutic potential. We performed a comprehensive characterization of Endosomal/lysosomal proton channel TMEM175 including:
1. LLM-powered literature research
Our custom-tailored LLM extracted and formalized all relevant information about the protein from a large set of structured and unstructured data sources and stored it in the form of a Knowledge Graph. This comprehensive analysis allowed us to gain insight into Endosomal/lysosomal proton channel TMEM175 therapeutic significance, existing small molecule ligands, relevant off-targets, and protein-protein interactions.
Fig. 1. Preliminary target research workflow
2. AI-Driven Conformational Ensemble Generation
Starting from the initial protein structure, we employed advanced AI algorithms to predict alternative functional states of Endosomal/lysosomal proton channel TMEM175, including large-scale conformational changes along "soft" collective coordinates. Through molecular simulations with AI-enhanced sampling and trajectory clustering, we explored the broad conformational space of the protein and identified its representative structures. Utilizing diffusion-based AI models and active learning AutoML, we generated a statistically robust ensemble of equilibrium protein conformations that capture the receptor's full dynamic behavior, providing a robust foundation for accurate structure-based drug design.
Fig. 2. AI-powered molecular dynamics simulations workflow
3. Binding pockets identification and characterization
We employed the AI-based pocket prediction module to discover orthosteric, allosteric, hidden, and cryptic binding pockets on the protein’s surface. Our technique integrates the LLM-driven literature search and structure-aware ensemble-based pocket detection algorithm that utilizes previously established protein dynamics. Tentative pockets are then subject to AI scoring and ranking with simultaneous detection of false positives. In the final step, the AI model assesses the druggability of each pocket enabling a comprehensive selection of the most promising pockets for further targeting.
Fig. 3. AI-based binding pocket detection workflow
4. AI-Powered Virtual Screening
Our ecosystem is equipped to perform AI-driven virtual screening on Endosomal/lysosomal proton channel TMEM175. With access to a vast chemical space and cutting-edge AI docking algorithms, we can rapidly and reliably predict the most promising, novel, diverse, potent, and safe small molecule ligands of Endosomal/lysosomal proton channel TMEM175. This approach allows us to achieve an excellent hit rate and to identify compounds ready for advanced lead discovery and optimization.
Fig. 4. The screening workflow of Receptor.AI
Receptor.AI, in partnership with Reaxense, developed a next-generation technology for on-demand focused library design to enable extensive target exploration.
The focused library for Endosomal/lysosomal proton channel TMEM175 includes a list of the most effective modulators, each annotated with 38 ADME-Tox and 32 physicochemical and drug-likeness parameters. Furthermore, each compound is shown with its optimal docking poses, affinity scores, and activity scores, offering a detailed summary.
Endosomal/lysosomal proton channel TMEM175
partner:
Reaxense
upacc:
Q9BSA9
UPID:
TM175_HUMAN
Alternative names:
Transmembrane protein 175
Alternative UPACC:
Q9BSA9; D3DVN4; Q8ND13
Background:
The Endosomal/lysosomal proton channel TMEM175, also known as Transmembrane protein 175, plays a pivotal role in maintaining pH homeostasis within endosomes and lysosomes. This protein functions as a proton-activated proton channel, facilitating proton efflux to regulate the steady-state pH. Additionally, TMEM175 may operate as a potassium channel, influencing potassium conductance. Its involvement in autophagosome-lysosome fusion underscores its importance in cellular processes.
Therapeutic significance:
TMEM175's dysfunction is linked to Parkinson disease, a neurodegenerative disorder characterized by motor and cognitive impairments. Variants affecting TMEM175 contribute to unstable lysosomal pH, impacting autophagosome clearance and mitochondrial respiration. Understanding TMEM175's role could pave the way for novel therapeutic strategies targeting lysosomal dysfunction in Parkinson's disease.
