AI-ACCELERATED DRUG DISCOVERY
Available from Reaxense
This protein is integrated into the Receptor.AI ecosystem as a prospective target with high therapeutic potential. We performed a comprehensive characterization of Eukaryotic translation initiation factor 2-alpha kinase 3 including:
1. LLM-powered literature research
Our custom-tailored LLM extracted and formalized all relevant information about the protein from a large set of structured and unstructured data sources and stored it in the form of a Knowledge Graph. This comprehensive analysis allowed us to gain insight into Eukaryotic translation initiation factor 2-alpha kinase 3 therapeutic significance, existing small molecule ligands, relevant off-targets, and protein-protein interactions.
Fig. 1. Preliminary target research workflow
2. AI-Driven Conformational Ensemble Generation
Starting from the initial protein structure, we employed advanced AI algorithms to predict alternative functional states of Eukaryotic translation initiation factor 2-alpha kinase 3, including large-scale conformational changes along "soft" collective coordinates. Through molecular simulations with AI-enhanced sampling and trajectory clustering, we explored the broad conformational space of the protein and identified its representative structures. Utilizing diffusion-based AI models and active learning AutoML, we generated a statistically robust ensemble of equilibrium protein conformations that capture the receptor's full dynamic behavior, providing a robust foundation for accurate structure-based drug design.
Fig. 2. AI-powered molecular dynamics simulations workflow
3. Binding pockets identification and characterization
We employed the AI-based pocket prediction module to discover orthosteric, allosteric, hidden, and cryptic binding pockets on the protein’s surface. Our technique integrates the LLM-driven literature search and structure-aware ensemble-based pocket detection algorithm that utilizes previously established protein dynamics. Tentative pockets are then subject to AI scoring and ranking with simultaneous detection of false positives. In the final step, the AI model assesses the druggability of each pocket enabling a comprehensive selection of the most promising pockets for further targeting.
Fig. 3. AI-based binding pocket detection workflow
4. AI-Powered Virtual Screening
Our ecosystem is equipped to perform AI-driven virtual screening on Eukaryotic translation initiation factor 2-alpha kinase 3. With access to a vast chemical space and cutting-edge AI docking algorithms, we can rapidly and reliably predict the most promising, novel, diverse, potent, and safe small molecule ligands of Eukaryotic translation initiation factor 2-alpha kinase 3. This approach allows us to achieve an excellent hit rate and to identify compounds ready for advanced lead discovery and optimization.
Fig. 4. The screening workflow of Receptor.AI
Receptor.AI, in partnership with Reaxense, developed a next-generation technology for on-demand focused library design to enable extensive target exploration.
The focused library for Eukaryotic translation initiation factor 2-alpha kinase 3 includes a list of the most effective modulators, each annotated with 38 ADME-Tox and 32 physicochemical and drug-likeness parameters. Furthermore, each compound is shown with its optimal docking poses, affinity scores, and activity scores, offering a detailed summary.
Eukaryotic translation initiation factor 2-alpha kinase 3
partner:
Reaxense
upacc:
Q9NZJ5
UPID:
E2AK3_HUMAN
Alternative names:
PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase
Alternative UPACC:
Q9NZJ5; A0AVH1; A0AVH2; B2RCU9; O95846; Q53QY0; Q53SB1
Background:
Eukaryotic translation initiation factor 2-alpha kinase 3, also known as PRKR-like endoplasmic reticulum kinase and Pancreatic eIF2-alpha kinase, plays a pivotal role in metabolic-stress sensing. It phosphorylates EIF2S1/eIF-2-alpha under stress conditions, initiating the integrated stress response (ISR) for adaptation to challenges such as unfolded protein response (UPR) and low amino acid availability. This kinase is a key activator in global protein synthesis inhibition and facilitates the preferential translation of ISR-specific mRNAs.
Therapeutic significance:
The protein's involvement in Wolcott-Rallison syndrome, a rare autosomal recessive disorder characterized by insulin-dependent diabetes and multisystem manifestations, highlights its therapeutic significance. Understanding the role of Eukaryotic translation initiation factor 2-alpha kinase 3 could open doors to potential therapeutic strategies for managing this syndrome and related metabolic and stress response disorders.
